5MWZ

Crystal structure of the human BRPF1 bromodomain in complex with BZ073

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000019
Spacegroup name	P 32 2 1
Unit cell lengths	60.811, 60.811, 63.207
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.460 - 1.250
R-factor	0.1647
Rwork	0.164
R-free	0.18070
RMSD bond length	0.004
RMSD bond angle	0.768
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.460
High resolution limit [Å]	1.250
Rmerge	0.058
Rpim	0.014
Number of reflections	37830
<I/σ(I)>	31.4
Completeness [%]	99.9
Redundancy	18.4
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M Bis-Tris Propane pH7.4, 25% PEG3350, 5% EG, 0.15 M NaNO3