5MWX

Galectin-1 in Complex with Ligand JB60

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97242
Spacegroup name	P 21 21 21
Unit cell lengths	43.260, 58.250, 111.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.319 - 1.290
R-factor	0.1706
Rwork	0.169
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	1.653
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_2443)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.320
High resolution limit [Å]	1.290
Number of reflections	71546
<I/σ(I)>	11.71
Completeness [%]	99.0
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.5 M AMMONIUM SULFATE, PH 7.5