5MWI
Crystal structure of G(CUG)8G duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-18 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 43.514, 43.514, 198.101 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.020 - 1.510 |
| R-factor | 0.19488 |
| Rwork | 0.192 |
| R-free | 0.25621 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e48 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.128 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.045 | |
| Number of reflections | 11846 | 1865 |
| <I/σ(I)> | 21.8 | 2.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.4 | 7.2 |
| CC(1/2) | 0.990 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | magnesium acetate, cacodylate and lithium sulfate |
