5MW9

Complex between the Leucine Zipper (LZ) and Centrosomin-motif 2 (CM2) domains of Drosophila melanogaster Centrosomin (Cnn) - L535E mutant form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97623
Spacegroup name	C 1 2 1
Unit cell lengths	275.792, 43.000, 48.350
Unit cell angles	90.00, 96.57, 90.00

Refinement procedure

Resolution	31.352 - 2.200
R-factor	0.2594
Rwork	0.258
R-free	0.30160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.311
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.355	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.067	0.747
Rpim	0.046	0.531
Number of reflections	27935	2406
<I/σ(I)>	6.7	1.5
Completeness [%]	96.4	96.7
Redundancy	2.9	2.9
CC(1/2)	0.993	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	18%(w/v) PEG2KMME, 0.2M sodium cacodylate pH6.5