5MW5
Human Jagged2 C2-EGF2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.3 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.266, 83.878, 99.352 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.091 - 2.700 |
R-factor | 0.2315 |
Rwork | 0.230 |
R-free | 0.26350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mv7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.686 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.900 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.107 | 0.559 |
Number of reflections | 11513 | |
<I/σ(I)> | 12.9 | 3.7 |
Completeness [%] | 99.6 | 100 |
Redundancy | 7.7 | 8.1 |
CC(1/2) | 0.996 | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | PEG 8000, potassium dihydrogen phosphate |