5MW5
Human Jagged2 C2-EGF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.3 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.266, 83.878, 99.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.091 - 2.700 |
| R-factor | 0.2315 |
| Rwork | 0.230 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mv7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.686 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.900 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.107 | 0.559 |
| Number of reflections | 11513 | |
| <I/σ(I)> | 12.9 | 3.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7.7 | 8.1 |
| CC(1/2) | 0.996 | 0.892 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG 8000, potassium dihydrogen phosphate |
