5MVW
Complex between the Leucine Zipper (LZ) and Centrosomin-motif 2 (CM2) domains of Drosophila melanogaster Centrosomin (Cnn)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.28229 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.880, 44.270, 72.530 |
| Unit cell angles | 90.00, 107.77, 90.00 |
Refinement procedure
| Resolution | 42.739 - 1.820 |
| R-factor | 0.2233 |
| Rwork | 0.222 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.559 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.070 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.077 | 3.048 |
| Rpim | 0.019 | 0.757 |
| Number of reflections | 24465 | 1806 |
| <I/σ(I)> | 22.9 | 1.1 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 16.9 | 17 |
| CC(1/2) | 1.000 | 0.574 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 20%(w/v) PEG6K, 0.1M Tris pH8.0, 0.2M MgCl2 |
