5MVK
Crystal structure of an unmodified A-DNA dodecamer containing 3 consecutive CpG steps
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.970 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 43.678, 43.678, 58.887 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.826 - 1.531 |
R-factor | 0.1809 |
Rwork | 0.180 |
R-free | 0.18770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.379 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.830 |
High resolution limit [Å] | 1.530 |
Number of reflections | 10206 |
<I/σ(I)> | 19.7 |
Completeness [%] | 99.9 |
Redundancy | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium chloride (12 mM) Potassium chloride (80 mM) Sodium cacodylate (40 mM, pH 7.0) Spermine tetrahydrochloride (12 mM) MPD (40% v/v) |