5MVJ
Structure of DC8E8 Fab at pH 6.5 crystallized in space-group P1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 61.220, 82.260, 89.310 |
Unit cell angles | 92.32, 95.47, 89.82 |
Refinement procedure
Resolution | 34.370 - 2.500 |
R-factor | 0.2219 |
Rwork | 0.219 |
R-free | 0.27210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4OZ4 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.616 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.370 | 34.370 | 2.560 |
High resolution limit [Å] | 2.500 | 11.180 | 2.500 |
Rmerge | 0.082 | 0.025 | 0.445 |
Rmeas | 0.113 | 0.035 | 0.614 |
Number of reflections | 58019 | 596 | 4296 |
<I/σ(I)> | 9.31 | 24.5 | 1.96 |
Completeness [%] | 96.7 | 88.8 | 96.7 |
Redundancy | 2.067 | 2.049 | 2.058 |
CC(1/2) | 0.990 | 0.997 | 0.607 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20% PEG 8000, 0.1 M Na-cacodylate, 0.2 M Mg-acetate |