5MV0
Structure of an N-terminal domain of a reptarenavirus L protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.970, 112.342, 153.848 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.730 - 1.930 |
R-factor | 0.175 |
Rwork | 0.173 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.865 |
Data reduction software | iMOSFLM (7.1.3) |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.730 | 2.000 |
High resolution limit [Å] | 1.931 | 1.931 |
Number of reflections | 74927 | 7396 |
<I/σ(I)> | 15.33 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 279 | 20% PEG 200, 2.5% PEG 3000, and 100 mM MES, pH 5.7 |