5MUZ

Structure of a C-terminal domain of a reptarenavirus L protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	74.830, 42.870, 70.830
Unit cell angles	90.00, 114.44, 90.00

Refinement procedure

Resolution	64.480 - 1.776
R-factor	0.217
Rwork	0.214
R-free	0.28080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mus
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	iMOSFLM (7.1.3)
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.480	1.840
High resolution limit [Å]	1.776	1.776
Rmerge	0.020
Rmeas	0.028
Number of reflections	19347	1919
<I/σ(I)>	7.41
Completeness [%]	97.0	97
Redundancy	1.9	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris, pH 7.9, 1.3 M trisodium citrate