5MUY
Structure of a C-terminal domain of a reptarenavirus L protein with m7GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.340, 76.601, 116.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.930 - 1.990 |
| R-factor | 0.2253 |
| Rwork | 0.223 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mus |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.935 |
| Data reduction software | iMOSFLM (7.1.3) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.930 | 2.061 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.148 | |
| Rmeas | 0.209 | |
| Number of reflections | 46169 | 4641 |
| <I/σ(I)> | 4.24 | |
| Completeness [%] | 97.0 | 97 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 37% Jeffamine ED-2001, 2 mM TCEP and 100 mM HEPES pH 7.1 |
