5MUX
Crystal structure of 2-methylcitrate dehydratase (MmgE) from Bacillus subtilis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.831, 194.409, 90.869 |
| Unit cell angles | 90.00, 92.74, 90.00 |
Refinement procedure
| Resolution | 30.800 - 2.000 |
| R-factor | 0.15279 |
| Rwork | 0.151 |
| R-free | 0.18926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.484 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.800 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 200076 |
| <I/σ(I)> | 4.5 |
| Completeness [%] | 99.5 |
| Redundancy | 6.3 |
| CC(1/2) | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M potassium/sodium tartrate, 20 % w/v PEG 3350. |
