5MUQ
Crystal structure of DC8E8 Fab at pH 7.0 containing a Zn atom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8166 |
| Spacegroup name | P 31 |
| Unit cell lengths | 113.510, 113.510, 69.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.300 - 2.620 |
| R-factor | 0.2156 |
| Rwork | 0.212 |
| R-free | 0.27940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 4OZ4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.732 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.300 | 2.670 |
| High resolution limit [Å] | 2.616 | 2.616 |
| Rmeas | 0.050 | 0.804 |
| Number of reflections | 29733 | |
| <I/σ(I)> | 28.47 | 3.16 |
| Completeness [%] | 98.5 | 80.6 |
| Redundancy | 5.8 | 4.9 |
| CC(1/2) | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 16% PEG 3350, 0.2 M imidazol, 0.02 M ZnSO4 |
