5MUM
Glycoside Hydrolase BACINT_00347
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 53.313, 68.438, 118.696 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.600 - 1.800 |
R-factor | 0.15353 |
Rwork | 0.152 |
R-free | 0.19096 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4irt |
RMSD bond length | 0.013 |
RMSD bond angle | 1.554 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 68.600 |
High resolution limit [Å] | 1.800 |
Number of reflections | 255841 |
<I/σ(I)> | 12 |
Completeness [%] | 98.4 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350 and 200 mM Tri-potassium Citrate |