5MUL
Glycoside Hydrolase BT3686 bound to Glucuronic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.01 |
| Spacegroup name | P 61 |
| Unit cell lengths | 120.178, 120.178, 52.651 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.750 - 1.390 |
| R-factor | 0.17397 |
| Rwork | 0.173 |
| R-free | 0.19423 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.508 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.750 |
| High resolution limit [Å] | 1.390 |
| Number of reflections | 436522 |
| <I/σ(I)> | 13.5 |
| Completeness [%] | 99.6 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350 and 200 mM Potassium Acetate |
