5MUI
Glycoside hydrolase BT_0996
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.180, 80.740, 51.410 |
Unit cell angles | 90.00, 114.99, 90.00 |
Refinement procedure
Resolution | 45.480 - 1.770 |
R-factor | 0.17597 |
Rwork | 0.174 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | BT0996 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.548 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.480 |
High resolution limit [Å] | 1.770 |
Number of reflections | 36153 |
<I/σ(I)> | 11.9 |
Completeness [%] | 99.8 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 3350 and 0.2 M Lithium Acetate |