5MUI
Glycoside hydrolase BT_0996
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.180, 80.740, 51.410 |
| Unit cell angles | 90.00, 114.99, 90.00 |
Refinement procedure
| Resolution | 45.480 - 1.770 |
| R-factor | 0.17597 |
| Rwork | 0.174 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BT0996 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.548 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.480 |
| High resolution limit [Å] | 1.770 |
| Number of reflections | 36153 |
| <I/σ(I)> | 11.9 |
| Completeness [%] | 99.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 3350 and 0.2 M Lithium Acetate |
