5MUH
Crystal structure of an amyloidogenic light chain dimer H7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.873 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.010, 72.820, 73.720 |
Unit cell angles | 90.00, 121.19, 90.00 |
Refinement procedure
Resolution | 55.450 - 2.650 |
R-factor | 0.208 |
Rwork | 0.205 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m6i |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.990 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.079 | 0.645 |
Number of reflections | 13069 | 1879 |
<I/σ(I)> | 7.6 | 1.6 |
Completeness [%] | 97.0 | 98.2 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH7.5, 10% 2-propanol, 20% w/v PEG 4K |