5MUH
Crystal structure of an amyloidogenic light chain dimer H7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.873 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.010, 72.820, 73.720 |
| Unit cell angles | 90.00, 121.19, 90.00 |
Refinement procedure
| Resolution | 55.450 - 2.650 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m6i |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.990 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.079 | 0.645 |
| Number of reflections | 13069 | 1879 |
| <I/σ(I)> | 7.6 | 1.6 |
| Completeness [%] | 97.0 | 98.2 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH7.5, 10% 2-propanol, 20% w/v PEG 4K |
