5MUG

Self-assembled alpha-Tocopherol Transfer Protein Nanoparticles Promote Vitamin E Delivery Across an Endothelial Barrier

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.007900
Spacegroup name	I 4 3 2
Unit cell lengths	168.259, 168.259, 168.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.572 - 2.420
R-factor	0.1916
Rwork	0.191
R-free	0.21170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oip
RMSD bond length	0.003
RMSD bond angle	0.680
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.572	48.572	2.570
High resolution limit [Å]	2.420	7.190	2.420
Rmerge	0.085	0.024	1.195
Rmeas	0.096	0.027	1.343
Total number of observations	139722
Number of reflections	29032	1109	4576
<I/σ(I)>	14	49.39	1.41
Completeness [%]	98.6	97	95.9
Redundancy	4.813	4.835	4.772
CC(1/2)	0.998	0.999	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	291.15	0.1 M Ammonium sulfate,10% w/v Polyethylene glycol 4,000