5MUE
Self-assembled alpha-Tocopherol Transfer Protein Nanoparticles Promote Vitamin E Delivery Across an Endothelial Barrier
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-31 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.99987 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 168.184, 168.184, 168.184 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.641 - 2.397 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.21520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oip |
RMSD bond length | 0.002 |
RMSD bond angle | 0.617 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.551 | 48.551 | 2.540 |
High resolution limit [Å] | 2.397 | 7.120 | 2.400 |
Rmerge | 0.091 | 0.023 | 1.543 |
Rmeas | 0.095 | 0.025 | 1.621 |
Number of reflections | 30125 | 700 | 2543 |
<I/σ(I)> | 20.8 | 71.31 | 1.54 |
Completeness [%] | 99.7 | 97.8 | 99.1 |
Redundancy | 10.744 | 9.097 | 10.692 |
CC(1/2) | 0.999 | 1.000 | 0.599 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 291.15 | 0.1 M Ammonium sulfate,10% w/v Polyethylene glycol 4,000 |