5MUD
Crystal structure of an amyloidogenic light chain dimer H6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97895 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 80.055, 72.810, 84.992 |
| Unit cell angles | 90.00, 105.29, 90.00 |
Refinement procedure
| Resolution | 52.980 - 2.340 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m6i |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.980 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.045 | 0.512 |
| Number of reflections | 19281 | 1908 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 96.0 | 95.8 |
| Redundancy | 3.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bicine pH 9.0, 2% w/v 1,4-Dioxane, 10% w/v PEG 20K |
