5MU1

UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum soaked with K2PtI6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-16
Detector	MAR CCD 130 mm
Wavelength(s)	1.07141
Spacegroup name	P 61
Unit cell lengths	116.950, 116.950, 301.491
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	101.280 - 3.480
R-factor	0.238
Rwork	0.237
R-free	0.25200
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	0.240
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	autoSHARP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	150.750	3.670
High resolution limit [Å]	3.480	3.480
Rmerge		3.573
Rmeas		3.730
Rpim		1.089
Number of reflections	28757	4354
<I/σ(I)>	15.4	1.3
Completeness [%]	96.2	100
Redundancy	23.2	23.3
CC(1/2)		0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	51.4 mM MES pH 3.3, 44.6 Imidazole pH 8.3, 20% PEG 8K, 10% Ethylene Glycole, 120 mM Morpheus Ethylene glycols mix