5MTZ

Crystal structure of a long form RNase Z from yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 41
Unit cell lengths	136.000, 136.000, 115.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.610 - 2.990
R-factor	0.1849
Rwork	0.181
R-free	0.26170
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.503
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.610	49.607	3.170
High resolution limit [Å]	2.990	8.840	2.990
Rmerge		0.059	2.274
Rmeas		0.063	2.437
Number of reflections	83709	3230	13304
<I/σ(I)>	7.52	29.94	0.83
Completeness [%]	99.7	99.5	98.4
Redundancy	7.8	7.757	7.629
CC(1/2)	0.991	0.997	0.381

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Hepes, pH 7.5, 25% (w/v) PEG 8000