5MTT

Maltodextrin binding protein MalE1 from L. casei BL23 bound to maltotetraose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	53.494, 83.079, 85.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.540 - 1.120
R-factor	0.12
Rwork	0.120
R-free	0.13700
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.082
Data reduction software	XDS (VERSION November 11, 2013)
Data scaling software	XDS (VERSION November 11, 2013)
Phasing software	SHELXDE (2013/2)
Refinement software	PHENIX ((1.10.1-2155_1168: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.280	1.158
High resolution limit [Å]	1.118	1.118
Rmerge		1.122
Rpim		0.386
Number of reflections	146247
<I/σ(I)>	23.52	2
Completeness [%]	99.7	97.76
Redundancy	11.6	11.6
CC(1/2)	1.000	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	0.1 mM Na-Hepes, pH 7.0, 20-25% Jeffamine ED2003, pH 7.0, 5 mM maltotetraose, 100 mM LiCl