5MTN
Monobody Mb(Lck_1) bound to Lck-Sh2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-06 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 91.397, 91.397, 88.778 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.700 - 2.850 |
R-factor | 0.22944 |
Rwork | 0.228 |
R-free | 0.26503 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.317 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.700 |
High resolution limit [Å] | 2.850 |
Number of reflections | 10316 |
<I/σ(I)> | 23.79 |
Completeness [%] | 100.0 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0,2M Lithium sulfate / 0.1M TRIS pH8.5 / 10% PEG8K + 10%PEG1K |