5MTM
Monobody Mb(Lck_3) bound to Lck-SH2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 81.834, 81.834, 105.961 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.917 - 2.405 |
R-factor | 0.2078 |
Rwork | 0.205 |
R-free | 0.25680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.159 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.920 |
High resolution limit [Å] | 2.400 |
Number of reflections | 14544 |
<I/σ(I)> | 37.91 |
Completeness [%] | 100.0 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M MES pH6.5, 15 % PEG 20000 |