5MTL
Crystal structure of an amyloidogenic light chain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 126.15 |
| Detector technology | PIXEL |
| Collection date | 2014-06-15 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.484, 65.085, 88.386 |
| Unit cell angles | 84.96, 86.66, 79.93 |
Refinement procedure
| Resolution | 29.289 - 2.450 |
| R-factor | 0.2363 |
| Rwork | 0.234 |
| R-free | 0.27680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m6i |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.570 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.126 | 0.610 |
| Number of reflections | 36581 | 3606 |
| <I/σ(I)> | 8.8 | 2.2 |
| Completeness [%] | 98.2 | 97.9 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Sodium cacodylate, 27% w/v PEG 2000 MME |
