5MSY
Glycoside hydrolase BT_1012
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 262.455, 262.455, 184.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.950 - 2.300 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kzs |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.519 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.950 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.970 | |
| Number of reflections | 106979 | |
| <I/σ(I)> | 10.8 | 2.5 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 10.7 | 11 |
| CC(1/2) | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 40 % (v/v) MPD, 0.2 M ammonium phosphate and Tris pH 8.5 |
