5MSN
Structure of the Dcc1 Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2014-07-31 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9200 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.682, 100.697, 83.495 |
Unit cell angles | 90.00, 100.67, 90.00 |
Refinement procedure
Resolution | 29.357 - 2.002 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.23010 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.592 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | autoSHARP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.360 | 2.074 |
High resolution limit [Å] | 2.000 | 2.002 |
Rmerge | 0.116 | 0.974 |
Number of reflections | 71317 | |
<I/σ(I)> | 8.43 | 1.31 |
Completeness [%] | 95.0 | 87 |
Redundancy | 4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M potassium sodium tartrate and 18% PEG 3,350 |