5MSJ
Mouse PA28alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.790, 119.800, 196.670 |
| Unit cell angles | 94.21, 98.52, 87.98 |
Refinement procedure
| Resolution | 29.650 - 3.500 |
| R-factor | 0.25122 |
| Rwork | 0.250 |
| R-free | 0.28279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1avo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.087 | 0.434 |
| Number of reflections | 92743 | |
| <I/σ(I)> | 9.1 | 1.9 |
| Completeness [%] | 93.1 | 93.3 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M Hepes, 25 % PEG 1000 |
