5MSE
GFP nuclear transport receptor mimic 3B8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.401, 73.398, 93.927 |
Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
Resolution | 46.860 - 1.660 |
R-factor | 0.17532 |
Rwork | 0.174 |
R-free | 0.20573 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ema |
RMSD bond length | 0.026 |
RMSD bond angle | 2.318 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.280 | 1.723 |
High resolution limit [Å] | 1.664 | 1.664 |
Rmerge | 0.129 | 1.910 |
Number of reflections | 114871 | |
<I/σ(I)> | 12.47 | 1.18 |
Completeness [%] | 98.4 | 96.49 |
Redundancy | 6.8 | 7 |
CC(1/2) | 0.998 | 0.468 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 5-9% PEG8000, 28% glycerol, 100?mM Imidazole (pH 7.1) |