5MS8

Crystal structure of the legionella pneumophila effector protein RavZ_1-487

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9780
Spacegroup name	I 4 2 2
Unit cell lengths	221.270, 221.270, 73.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.047 - 2.850
R-factor	0.2036
Rwork	0.201
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cqc
RMSD bond length	0.009
RMSD bond angle	1.452
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.050	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.216	1.927
Number of reflections	21565
<I/σ(I)>	14.09	1.85
Completeness [%]	100.0	100
Redundancy	26.7	27.9
CC(1/2)	0.998	0.134

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277.15	16% PEG3350, 0.2 M BaCl2 and 0.1 M MES