5MS7
Crystal structure of the legionella pneumophila effector protein RavZ_20-502
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97862 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 222.770, 222.770, 72.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.145 - 2.800 |
| R-factor | 0.2091 |
| Rwork | 0.207 |
| R-free | 0.25350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cqc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.379 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.150 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.194 | 1.951 |
| Number of reflections | 22921 | |
| <I/σ(I)> | 15.14 | 1.94 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 26.7 | 26.3 |
| CC(1/2) | 0.999 | 0.165 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277.15 | 16% PEG3350, 0.2 M BaCl2 and 0.1 M MES |
