5MS6

High-salt structure of RavZ LIR2-fused human LC3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99992
Spacegroup name	P 43 21 2
Unit cell lengths	69.300, 69.300, 117.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 1.900
R-factor	0.2082
Rwork	0.206
R-free	0.26090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z0d
RMSD bond length	0.008
RMSD bond angle	1.169
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.203	1.551
Number of reflections	23355
<I/σ(I)>	14.37	1.52
Completeness [%]	100.0	100
Redundancy	23.9	15.2
CC(1/2)	0.999	0.203

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1 M sodium acetate and 3 M NaCl