5MS5

Low-salt structure of RavZ LIR2-fused human LC3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99992
Spacegroup name	P 43 21 2
Unit cell lengths	69.610, 69.610, 117.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.222 - 1.530
R-factor	0.2096
Rwork	0.208
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z0e
RMSD bond length	0.008
RMSD bond angle	1.179
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.222	1.585
High resolution limit [Å]	1.530	1.530
Rmerge	0.177	2.205
Number of reflections	44250
<I/σ(I)>	14.69	1.42
Completeness [%]	100.0	100
Redundancy	25.4	24.6
CC(1/2)	0.999	0.165

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M citric acid anhydrous and 1.6 M ammonium sulfate