5MS2

Crystal structure of the Legionella pneumophila effector protein RavZ in complex with human LC3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-03
Detector	PSI PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	51.550, 69.550, 90.170
Unit cell angles	90.00, 101.08, 90.00

Refinement procedure

Resolution	48.080 - 2.470
R-factor	0.1805
Rwork	0.177
R-free	0.23840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cqc 2z0d
RMSD bond length	0.010
RMSD bond angle	1.446
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.080	2.560
High resolution limit [Å]	2.470	2.470
Rmerge	0.180	0.720
Number of reflections	22607
<I/σ(I)>	9.51	2.57
Completeness [%]	99.9	99.42
Redundancy	6.8	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277.15	0.17 M ammonium acetate, 0.085M tri sodium citrate, 25.5% PEG 4000 and 15% glycerol