5MRX
27-nt SRL with a 5-hydroxymethyl cytidine modification
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-27 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.77 |
Spacegroup name | P 43 |
Unit cell lengths | 29.510, 29.510, 76.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.510 - 0.851 |
R-factor | 0.1587 |
Rwork | 0.145 |
R-free | 0.16820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dvz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.123 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 0.850 |
Rmeas | 0.045 |
Number of reflections | 57106 |
<I/σ(I)> | 30.32 |
Completeness [%] | 99.6 |
Redundancy | 22.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | well: 3.0M (NH4)2SO4 drop: 50mM KMops pH7.0, 10mM MnCl2, 10mM MgCl2, 1.0mM Na-EDTA pH 8.0, 10mM Tris pH 8.0, RNA 2.5mg/ml 2vol drop + 1vol well |