5MRT
Crystal structure of L5 protease Lysobacter sp. XL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-27 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.54179 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.549, 66.668, 96.642 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.334 - 1.600 |
R-factor | 0.1704 |
Rwork | 0.168 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mrr |
RMSD bond length | 0.006 |
RMSD bond angle | 0.911 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 53331 | |
<I/σ(I)> | 9.7 | 1.8 |
Completeness [%] | 98.6 | 97.7 |
Redundancy | 5.9 | 3.46 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2,7M Na formate, 0,2 M ammonium acetate, PIPES |