5MR9
Ligand-receptor complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.992 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 58.080, 32.750, 61.910 |
| Unit cell angles | 90.00, 107.06, 90.00 |
Refinement procedure
| Resolution | 48.150 - 2.400 |
| R-factor | 0.25 |
| Rwork | 0.248 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.060 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 8997 |
| <I/σ(I)> | 6.7 |
| Completeness [%] | 99.9 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 10.5 | 293 | 40% MPD and 100 mM CAPS pH 10.5. |
