5MQQ
Transcriptional repressor AmtR of corynebacterium glutamicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-04 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 149.783, 160.638, 51.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.240 - 2.093 |
R-factor | 0.2112 |
Rwork | 0.209 |
R-free | 0.24970 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.939 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.240 | 2.220 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.086 | 0.751 |
Number of reflections | 73411 | |
<I/σ(I)> | 13.5 | 2.6 |
Completeness [%] | 98.8 | 95.9 |
Redundancy | 4.05 | 3.86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | AmtR (12 mg/ml) in 20 mM Tris HCl, pH 8.0, 0.2 M NaCl Crystals obtained under the conditions: 0.1 M Bis-Tris pH 5.8, 20 % PEG 3350 and 0.2 M (NH4)2SO4 with a protein to drop ratio of 1:1 microliters |