5MQI

Crystal structure of the N-terminal domain of human Timeless

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2014-09-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978570
Spacegroup name	C 2 2 21
Unit cell lengths	68.008, 71.337, 185.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.456 - 1.847
R-factor	0.1716
Rwork	0.170
R-free	0.19740
RMSD bond length	0.015
RMSD bond angle	1.288
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	1.890
High resolution limit [Å]	1.847	1.850
Rmerge	0.033	0.752
Number of reflections	36572
<I/σ(I)>	18.6	1.6
Completeness [%]	94.4	87.8
Redundancy	3.8	3.7
CC(1/2)	0.999	0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	reservoir solution: 20% (w/v) PEG 3350, 200 mM Na2SO4 protein solution: 8.5 mg/mL, 150 mM KCl, 25 mM Heps pH 7.2, 1 mM TCEP 200 nL protein solution and 200 nL of reservoir solution were mixed.