5MPX
Crystal structure of Arabidopsis thaliana RNA editing factor MORF1, space group P2(1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.771 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.892, 69.818, 69.324 |
| Unit cell angles | 90.00, 108.95, 90.00 |
Refinement procedure
| Resolution | 34.807 - 1.938 |
| R-factor | 0.1631 |
| Rwork | 0.163 |
| R-free | 0.19170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mpw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.830 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.938 | 1.940 |
| Number of reflections | 44847 | |
| <I/σ(I)> | 11.8 | 2.32 |
| Completeness [%] | 97.7 | 83.9 |
| Redundancy | 6.1 | 4.7 |
| CC(1/2) | 0.569 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0,1 M Bicine, pH 9.0 and 2.4 M ammonium sulfate |
