5MPR
Single Amino Acid Variant of Human Mitochondrial Branched Chain Amino Acid Aminotransferase 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.032, 84.032, 105.446 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.520 - 1.600 |
| R-factor | 0.13052 |
| Rwork | 0.128 |
| R-free | 0.17939 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.816 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 57390 |
| <I/σ(I)> | 11.8 |
| Completeness [%] | 99.7 |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 293 | 0.2 M Sodiumfluoride, 0.1 M Bis Tris propane pH 6.5, 20% w/v Polyethylene glycol 3350 |
