5MPM
SERCA2a from pig heart
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2014-09-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.349, 253.287, 64.981 |
| Unit cell angles | 90.00, 100.86, 90.00 |
Refinement procedure
| Resolution | 63.320 - 3.300 |
| R-factor | 0.2083 |
| Rwork | 0.204 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fgo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.431 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.320 | 3.350 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.130 | |
| Rmeas | 0.155 | |
| Rpim | 0.082 | |
| Number of reflections | 25135 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 292.15 | 80 mM KCl, 15 mM MgCl2, 2 mM EGTA, 10 mM NaF, 200 uM CPA |
