5MPI
Structural Basis of Gene Regulation by the Grainyhead Transcription Factor Superfamily
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 104.405, 104.405, 116.254 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.620 - 2.345 |
| R-factor | 0.2235 |
| Rwork | 0.222 |
| R-free | 0.25150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.124 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.620 | 2.429 |
| High resolution limit [Å] | 2.345 | 2.345 |
| Rmerge | 0.119 | 3.394 |
| Number of reflections | 16189 | |
| <I/σ(I)> | 20.65 | 1.15 |
| Completeness [%] | 99.6 | 97.98 |
| Redundancy | 15.7 | 15.8 |
| CC(1/2) | 1.000 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 2.1 M (NH4)2SO4 and 0.1 M NaOAc, pH 5.5 |
