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5MPI

Structural Basis of Gene Regulation by the Grainyhead Transcription Factor Superfamily

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsBESSY BEAMLINE 14.1
Synchrotron siteBESSY
Beamline14.1
Temperature [K]100
Detector technologyPIXEL
Collection date2016-01-12
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.9184
Spacegroup nameP 61 2 2
Unit cell lengths104.405, 104.405, 116.254
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution47.620 - 2.345
R-factor0.2235
Rwork0.222
R-free0.25150
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.011
RMSD bond angle1.124
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]47.6202.429
High resolution limit [Å]2.3452.345
Rmerge0.1193.394
Number of reflections16189
<I/σ(I)>20.651.15
Completeness [%]99.697.98
Redundancy15.715.8
CC(1/2)1.0000.599
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP5.52772.1 M (NH4)2SO4 and 0.1 M NaOAc, pH 5.5

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