5MPF
Structural Basis of Gene Regulation by the Grainyhead Transcription Factor Superfamily
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98141 |
| Spacegroup name | P 41 |
| Unit cell lengths | 59.034, 59.034, 198.854 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.085 - 2.918 |
| R-factor | 0.2303 |
| Rwork | 0.228 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.665 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.090 | 3.022 |
| High resolution limit [Å] | 2.918 | 2.918 |
| Rmerge | 0.115 | |
| Number of reflections | 14650 | |
| <I/σ(I)> | 10.61 | 1.45 |
| Completeness [%] | 99.2 | 98.28 |
| Redundancy | 4.1 | 4.1 |
| CC(1/2) | 0.997 | 0.698 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | 10% (v/v) isopropanol and 0.1 M citric acid, pH 4.0 |
