5MP8
Crystal Structure of CK2alpha with ZT0432 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-25 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8726 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 65.170, 68.340, 331.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.160 - 1.920 |
R-factor | 0.213 |
Rwork | 0.212 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cvh |
RMSD bond length | 0.012 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.160 | 1.989 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.031 | 0.560 |
Number of reflections | 52615 | |
<I/σ(I)> | 12.6 | 1.1 |
Completeness [%] | 92.0 | 88 |
Redundancy | 1.9 | 1.8 |
CC(1/2) | 0.999 | 0.615 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 112.5mM Mes pH 6.5, 35% glycerol ethoxylate, 180 mM ammonium acetate |