5MP0
Human m7GpppN-mRNA Hydrolase (DCP2, NUDT20) Catalytic Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.050, 60.570, 65.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.730 - 1.630 |
| R-factor | 0.21441 |
| Rwork | 0.212 |
| R-free | 0.26178 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j3t |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.028 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.730 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmeas | 0.088 | 1.895 |
| Number of reflections | 24476 | |
| <I/σ(I)> | 11.3 | 0.8 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 0.1 M acetate pH 4.5 25% PEG3350 |
