5MOJ
Crystal structure of IgE-Fc epsilon 3-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.532, 104.065, 52.937 |
| Unit cell angles | 90.00, 101.53, 90.00 |
Refinement procedure
| Resolution | 51.867 - 2.260 |
| R-factor | 0.2032 |
| Rwork | 0.202 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ha0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.767 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.870 | 2.330 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.130 | 1.834 |
| Number of reflections | 23131 | |
| <I/σ(I)> | 5.9 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.1 | 4.2 |
| CC(1/2) | 0.993 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 25% PEG 4000 0.15M Ammonium Sulphate 0.1M MES |
