5MOH
Crystal structure of CK2alpha with ZT0583 bound.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-14 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 0.9600 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.663, 45.659, 63.833 |
Unit cell angles | 90.00, 111.70, 90.00 |
Refinement procedure
Resolution | 59.310 - 1.380 |
R-factor | 0.1969 |
Rwork | 0.197 |
R-free | 0.20260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cvh |
RMSD bond length | 0.012 |
RMSD bond angle | 1.050 |
Data reduction software | XDS ((VERSION January 10) |
Data scaling software | Aimless (0.2.14) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.310 | 1.390 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.045 | 0.385 |
Number of reflections | 58088 | |
<I/σ(I)> | 13.9 | 2.1 |
Completeness [%] | 90.5 | 45.5 |
Redundancy | 3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 107mM Mes pH 6.5, 29% glycerol ethoxylate, 1 M ammonium acetate |