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5MN9

Crystal structure of MINDY-1 tMIU in complex with K48-diUb

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE MASSIF-1
Synchrotron siteESRF
BeamlineMASSIF-1
Temperature [K]100
Detector technologyPIXEL
Collection date2015-05-01
DetectorDECTRIS PILATUS3 2M
Wavelength(s)0.96769
Spacegroup nameI 41
Unit cell lengths55.140, 55.140, 105.510
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution48.870 - 2.050
R-factor0.18484
Rwork0.183
R-free0.22341
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ubq
RMSD bond length0.010
RMSD bond angle1.441
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0155)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.8702.110
High resolution limit [Å]2.0502.050
Rmerge0.0430.544
Number of reflections9899
<I/σ(I)>19.42.9
Completeness [%]100.0100
Redundancy6.66.6
CC(1/2)0.9990.818
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.4293.15100 mM sodium acetate pH 5.4 and 18.5 % PEG3350

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