5MN9
Crystal structure of MINDY-1 tMIU in complex with K48-diUb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-01 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.96769 |
Spacegroup name | I 41 |
Unit cell lengths | 55.140, 55.140, 105.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.870 - 2.050 |
R-factor | 0.18484 |
Rwork | 0.183 |
R-free | 0.22341 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.441 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.870 | 2.110 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.043 | 0.544 |
Number of reflections | 9899 | |
<I/σ(I)> | 19.4 | 2.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.6 | 6.6 |
CC(1/2) | 0.999 | 0.818 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293.15 | 100 mM sodium acetate pH 5.4 and 18.5 % PEG3350 |